The NCI TranscriptionalPharmacodynamicsWorkbench

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About
Query for a Single Gene
Correlation Analysis
Time Course Graphs
Pathway Analysis
Transcription Factors
Receptors
User defined genes

The NCI Transcriptional Pharmacodynamics Workbench website has been developed using advanced computational and visualization tools for empowering developmental therapeutics investigators worldwide and providing them access to the genome-wide characterization of the NCI-60 cell lines and the enormous time-course databases on genome-wide response to treatment with drugs.

Principal Architect: Dr. Richard Simon
Development team: Drs. Yingdong Zhao, Eric Polley, Ming-Chung Li, Jianwen Fang, Xiaosheng Wang, Alida Palmisano, Peter Szabo; Mr. Henry Rivera
For questions or assistance, please contact the Support Team at ncitpwsupport@mail.nih.gov
Documentation: NCI Transcriptional Pharmacodynamics Workbench User Manual

When publishing results based on the NCI TP Workbench, please cite:
Monks, A, et al. "The NCI Transcriptional Pharmacodynamics Workbench: a tool to examine dynamic expression profiling of therapeutic response in the NCI-60 cell line panel." Cancer Research (2018): canres-0989.
https://www.ncbi.nlm.nih.gov/pubmed/30355619

** October 2021 **

TPWshiny: an interactive RShiny app to explore the therapeutic response of NCI-60 cell lines

TPWshiny is a powerful standalone visualization tool for the genome-wide characterization of NCI-60 human cell lines and response to 15 different anticancer drugs at different time points. TPWshiny is an easy to install, R Shiny application to facilitate more interactive exploration of data from the NCI Transcriptional Pharmacodynamics Workbench (NCI TPW).
Access or download TPWshiny at https://brb.nci.nih.gov/TPWshiny/.

Drugs	azacytidine, bortezomib, cisplatin, dasatinib, doxorubicin, erlotinib, geldanamycin, gemcitabine, lapatinib, paclitaxel, sirolimus, sorafenib, sunitinib, topotecan, vorinostat
Concentration	baseline, low concentration, high concentration
Time point	2 hr, 6 hr, 24 hr
Number of data points in Gene Expression experiments	169,817,571
Number of arrays	7623
Number of probe sets per chip	22277
Number of genes per chip	12704
Number of GI50 measurements	866
Number of genes with mutation data	1227
Number of genes with protein expression data	154
Number of pathways	65
Number of transcription factors	121
Number of groups of receptors	53

Heatmap for a cluster of genes

Expression of genes most correlated with:

Select drug:
Select time point:
Select drug concentration:
Input number of genes:

Enter gene symbol:

Query for a single gene's base line expression profile

Query for a single gene's expression profile treated with a specific drug

Select drug:

Time profiles for a single gene for multiple drugs
Static Graphs

Dynamic Graphs (requires Chrome or Firefox)

Select drug: ; Select drug concentration:

Top 50 genes with largest average fold change across cell lines and time points

Top 50 genes whose expressions are most correlated with GI50

Heatmap for receptor genes

Select group of receptors:

Select drug:

Sort cell lines by drug sensitivity (Log GI50)

Query for expression profile treated with a specific drug from a user defined gene list

Select drug:

Enter gene symbols: (one gene per line, maximum number of genes is 300)

Sort cell lines by drug sensitivity (Log GI50)

Heatmap for genes in a specific pathway

Select pathway: ; Select drug:

Sort cell lines by drug sensitivity (Log GI50)

Heatmap for target genes of a specific transcription factor

Select transcription factor: ; Select drug:

Heatmap of drug effects on expression of all transcription factors

Select drug:

Heatmap of drug effects on expression of a specific transcription factor

Select transcription factor: